OMNIeDNA Multi Rack - environmental DNA sample tube and cartridge holder
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Rack for holding samples while in the laboratory or in the field, greatly reducing the chances of spilling something
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The modular, versatile, open-source eDNA sampler to maximize detection and minimize complexity
The OMNIeDNA sampler is a modular environmental DNA (eDNA) concentration system built around a standard 50 mm cotton swab pad secured within a threaded connection ring. By placing this simple, low-cost membrane at the point of water entry, the OMNIeDNA filter system creates a consistent, focused filtration interface that works across virtually any water sampling scenario. Yes, you can also use more traditional filter membranes (48-52 mm diameter) if you feel so inclined!
Whether deployed passively or with minimal active force, the system directs water through the cotton membrane, capturing cellular material and extracellular DNA efficiently on a substrate that is both widely available and easily processed for downstream DNA extraction. The threaded ring architecture allows users to adapt the same filtration core to a wide range of sampling containers, flow conditions, or collection methods, making affordable ecosystem-wide surveying accessible in diverse environments. The OMNIeDNA filter system is an inexpensive tool that puts the power of eDNA sampling into the hands of non-specialist users. Simple materials, limitless adaptability.
For more information:
https://github.com/pknichols/OMNIeDNA
https://pknichols.com/research/simplifying-edna/
All components have been successfully printed on a Prusa MK4S using the following parameters:
Filament: 1.75mm PETG or ABS plastic (recommended over PLA for toughness, frequent use in harsh environments). ABS components can be smoothed with an Acetone vapor bath to facilitate routine bleach decontamination.
Infill: Gyroid, 30%+ (superior strength-to-weight ratio using less material, reducing print costs)
Layer height: 0.20 mm
Minimum perimeters per layer: 4
Print temperature: 220°C – 260°C (PETG/ABS);
Heated bed: 70 – 80°C (PETG); 90 – 110°C (ABS; enclosure and ventilation recommended)
Brim/supports: 8-10mm brim when using ABS; supports were only needed for printing the Canopy_bulkhead
While these settings are optimized for PETG/ABS, users should conduct test prints to validate dimensional accuracy and layer adhesion with their chosen filament.