Magnetic Separation Rack for PCR Tubes (8-strips & single tubes) – for magnetic bead protocols
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Magnetic bead separation rack for 0.2 ml PCR tubes (8-strips or singles), with glued-in NdFeB magnets.
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- Chemistry & Biology
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- Creative Commons — Attribution — Noncommercial
Magnetic separation rack for 0.2 mL PCR tubes, for bead-based clean-ups and size selection (SPRI/AMPure-type protocols, NGS library prep, magnetic bead DNA/RNA purification, etc.). It holds 8-tube strips or single tubes. It is a 3D-printed alternative to commercial racks such as the NEBNext Magnetic Separation Rack or the SeekGene magnetic racks.
The footprint is 30 x 96 mm. The positions are labelled 1-8. The labels are raised 0.6 mm and are separate objects in the 3MF files so that you can print them in a second colour. A rack ID letter (A–D) lets you tell several racks apart.
Two versions, depending on your tubes:
V1.2 (height: 23.2 mm) is for standard 8-strip packs with separate caps. Example: Sarstedt PCR 8-strip, 200 µl, art. no. 72.985.002
https://www.sarstedt.com/de/DEU/c/life-science/pcr-und-molekularbiologie/pcr-ketten/p/pcr-ketten-mit-separatem-deckel/72.985.002V1.3 “small” (height 21.2 mm) is for low-profile 8-strips. Example: Thermo Scientific Low Profile Tubes, art. no. AB0771
https://www.thermofisher.com/order/catalog/product/AB0771
Files:
PCR-Magnetic-Rack-V1_2-A/B/C/D.3mf and PCR-Magnetic-Rack-V1_3-small-A/B/C/D.3mf are the complete racks with labels, ready for multicolour printing. The four variants differ only in the rack ID letter.
PCR-Magnetic-Rack-V1_2-no-labels.stl and PCR-Magnetic-Rack-V1_3-small-no-labels.stl are single-colour versions without labels. They include all magnet pockets.
PCR-Magnetic-Rack-V1_2-no-labels-no-side-mags.stl and PCR-Magnetic-Rack-V1_3-small-no-labels-no-side-mags.stl are the same without the side magnet pockets. [One line on when to choose this version.]
Magnets (NdFeB N50, from magnethandel.de)
Neodymium magnets 6 x 4 x 2 mm, 8 pieces per rack
Neodymium magnets 12 x 6 x 2 mm, 4 pieces per rack
Assembly
The magnets are glued in after printing, so no print pause is needed. Use a strong glue such as two-component epoxy or cyanoacrylate. Insert all magnets with the same pole facing the tubes. Check this with a spare magnet before glueing, and mark the orientation on the first one. These are strong magnets. Keep them away from pacemakers, credit cards and other magnets while you work, because they can pinch.
Printing material is a matter of preference; I've printed with PLA.