Chamber Slide (Nunc Lab-Tek II) Microplate Adapter
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This is a simple adapter to use Nunc Lab-Tek II chambered coverglasses in any microscope or live cell imager that…
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This is a simple adapter to use Nunc Lab-Tek II chambered coverglasses in any microscope or live cell imager that accepts standard microplates. It is shown in the photo with Nunc cat # 155382, but any product in the same product series should work perfectly. Works very nicely in my laboratory's Cytation 5.
The tolerances make for a nice tight fit with my printer, filament and printing profiles, although adjusting horizontal expansion may be required in your setup. However, even if you can print this to get a nice tight fit for the chamber, I would still recommend 'locking' the chamber in place with some plasticine or Blu-Tack. This will help ensure there is no movement when imaging cells, and so that the chamber cannot 'jump' if the adapter is knocked.
If you use this design in a scientific manuscript, I only ask that you please state the design was received from Dr. Adrian West, cite this webpage, and send me a link to your published paper!
Print Settings
Printer: Ultimaker S3
Rafts: No
Supports: No
Resolution: 0.1 mm
Infill: 25% Grid
Filament: BASF Ultrafuse PET Natural
Very easy print. No brim needed when printed in PET on Ultimaker glass at 70°C with Magigoo adhesive, although I suspect you would probably need to use a brim for any other filament.