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Single chamber meshed 600mL Becker size holding chamber for patch clamp — image 1
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Single chamber meshed 600mL Becker size holding chamber for patch clamp

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Single chamber meshed 600mL Becker size holding chamber for patch clamp.

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Single chamber meshed 600mL Becker size holding chamber for patch clamp experiment. Allow top  and bottom perfusion of the sections for curing in bubbling artificial cerebrospinal fluid (during biocytin spread for example).

Use 1mm cut tubbing glued in a ring shape has a gasket to fit the setup in a standard 600mL Becker. The setup should then be slide near bottom using pure water and be pinned in place and so that it doesn't float when the ACSF is added.

The mesh was cut to size and glued on the edges.

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