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Parametric Agarose Gel Comb — image 1
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Parametric Agarose Gel Comb

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Customizable comb for agarose gels. This could also be customized for PAGE gels.

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Model information

License
Creative Commons — Attribution — Noncommercial

Customizable comb for agarose gels. The included STL is a BA-14 1.5mm thickness. However if you have the measurements for any comb, download the Fusion 360 file.

 

Once in Fusion 360 you can customize the parameters to fit any desired configuration. I run a teaching lab and was shocked that combs cost ~$80 each so I designed this to print for a few cents.

 

I have tested it with PLA and they work remarkably well if the agarose is poured at ~50°C. If you wanted to be extra sure they can stand the heat you could try printing in PETG.

 

Variables to Specify in Fusion360:

  1. NumTeeth: Number of wells
  2. ToothGroupLength: length from the left of the first tooth to the right of the last tooth
  3. ToothThickness: The thickness of the tooth (i.e. 1.0 or 1.5mm)
  4. BaseLength: length of the entire comb including the slots at the end which hang on the casting tray
  5. GapWidth: The gap between the wells. 1.831mm seems to be the standard, I would leave this value alone.