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Acute brain slice incubator — image 1
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Acute brain slice incubator

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To temporarily preserve acute brain slices in carbogen-bubbled aCSF

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Creative Commons — Attribution — Noncommercial

Update July 11, 2024: 

Made a newer version: https://www.printables.com/model/940315-acute-brain-slice-incubator_mk2 

 

See it in action: https://youtube.com/shorts/LdF34IaBJiM?si=LkmfdzMZeGoAy-1a

This was designed to temporarily preserve acute brain slices in carbogen-bubbled aCSF (Artificial cerebrospinal fluid). A similar commercial product costs over 300 USD (ridiculous). 

A piece of mesh/fabric is required between the top and main parts. 

The visor is to prevent bubbles from blowing brain slices away. 

The dimensions were based on a beaker I have by hand. I also uploaded the Fusion 360 file if you want to modify the leg length.