Re-Usable Slide Chamber for Immunocytochemistry / Cytospin
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A re-usable apparatus for ICC / cytospin to reduce plastic waste and reduce cost!
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Updated at source:
Model information
- Source
- Printables
- Category
- Chemistry & Biology
- License
- Creative Commons — Attribution
2024.05.17 Update
‘Optimal’ filaments for printing the ICC chamber capable of withstanding several days in the incubator are the following:
- ABS / ASA (Validated)
- PA-CF (Validated)
The following was printed using Polymaker ASA. Post-processing included sanding and smoothing using acetone to fuse layer lines. The chamber was tested by filling each well with 500ul of PBS and incubated for 1 week after which the volume of each well was measured. Approximately 40-50ul was lost from each well.
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Objective: Create a reusable slide chamber for cell culture and subsequent immunostaining.
Limitations with commercial slide chambers:
i) One-time use (plastic waste)
ii) Cost
Using a printed shell and silicone insert, this designs allows for repeated use and can be sterilized using EtOH.